Preparation of Samples for Aflatoxin Testing

Preparation of Samples for Aflatoxin Testing

 Aflatoxin toxins are produced by various types of fungi that can grow in foods such as pistachio nuts, nuts, grains, dried fruits, etc. More information in this article can be used to test other mycotoxins and ochratoxins.

 

Permitted levels of this toxin are very low - usually 5 ppb or less. Analysis was performed to ensure that these restrictions were not violated, and the sampling and testing process is regulated by law in most countries. However, test correction has not been combined with any standard method.

 

A study conducted by the authorities has found that the preparation of samples by scurrying offers significant benefits in addition to drying.

 

 Wahal Engineers high shear mixer blends have been used in this study, and it has been found that the aflatoxin content of repeated samples prepared by scurrying was significantly similar to those prepared by dry grinding. Wahal Engineer compounds are now used as a standard method in several countries.

The process

As mentioned above, in the absence of a standard procedure, there are many variations in the effectiveness of the preparation (or extraction) process, depending on the method and type of equipment used.
The volumes used for sample preparation will also vary depending on the product being tested. 
65 - 90 lbs samples are rare.

The usual procedure would be as follows:

The problem

Sample preparation is subject to several issues:

Effective reduction of particle size and sample homogenization before extraction is essential for accurate and repetitive results.

Other methods of dispersing solid objects, especially on a small scale in the laboratory are difficult to verify and can lead to discrepancies between clusters.

Milling machines may be difficult to clean, exacerbating potential pollution/hygiene issues.

The output phase is subject to a lot of variabilities due to the methods used, as described in the previous diagram. Once again it is difficult to confirm.

Dry digestion can cause severe heat loss.

The viscosity varies greatly from liquid to liquid.

Solution

Experiments have found that the preparation of samples by brushing using tools such as the Wahal Engineer high shear mixer offers significant advantages over dry grinding:

• Very low CV (coefficient of variation) is available.

• The particle size can be reached.

• Better homogenization of samples.

• Consistent effects throughout the viscosity range.

• Items of various sizes can be processed.

 

This efficiency and consistency between clusters are based on

Wahal Engineer's head mixing function, which works with the principle of the rotor-stator as follows:

Stage 1 Rotating a large rotary speed inside the working head creates a strong absorption of fluid and firms up

to the working head. Stage 2 The Centrifugal force drives the material toward the surrounding surface of the work surface where

they are under the grinding action where it is allowed between the tips of the rotor blades and the

inner wall of the stator. Stage 3 The solids are extracted by the stator and produced back in the body of the mixture. With a short

cycle of mixing all the contents of the vessel beyond the subject matter, it gradually reduces the

particle size to form a perfectly consistent product.

 

Benefits

• Reduced cleaning requirements.

• Processing times are greatly reduced.

• Laboratory units allow for accurate prediction of the performance of large units when enlarged.

 

 

Wahal Engineer manufactures laboratory and production mixes suitable for this application:

 

Laboratory Materials

• Suitable for samples of up to 3 kilos (6.5 lbs)

• Special Duplex Disintegrating application titles are used for this application; other alternate headers are available, allowing the unit to modify the list of mixing applications in the laboratory

• Several instrumentation options are available, including tachometers, ammeters, printers, website programs, or

• other computer-controlled devices.

In Tank Batch  Mixer homogenizer

 

High Shear Batch Mixers

• Suitable for batches up to 400 liters the US

• Can be used on mobile tents

• It can be easily moved from ship to ship